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Project Number page
Summary 20- 1
00030-14 Antigen Recognition and Activation of Immuno-
competent Cells. Paul 20-10
00035-07 Specificity in Immune Responses. Inman 20-11
00036-17 Immunoglobulin Genetics: Ontogeny and Differentia-
tion of Cells of the Rabbit Immune System. Mage 20-12
00037-15 Immunogenetics of Mouse Immunoglobulins and Gene-
tic Control of Antibody Response. Lieberman 20-13
00038-10 Structure and Activity Studies on Immunologi-
cally Important Cells and Proteins. Waxdal 20-14
00040-08 Genetic Control of Immunocompetent Cell Inter-
actions. Shevach 20-15
00147-07 The Mechanism of Activation of Buy Voltaren Emulgel Thymus-Derived
Lymphocytes. Schwartz 20-16
00148-07 Lymphocyte Interactions, Receptors and Functions.
00223-01 Cellular Interactions in Voltaren Emulgel Uk the Immune Response.
00224-01 Monoclonal Antibodies as Probes for Understand-
ing Cell Activation. Shevach 20-19
00225-01 The Effects of Lymphokines on Non-Lymphoid
Tissues. Green 20-20
00226-01 Rabbit Ig Allotypes: Structure, Voltaren Emulgel Cream Organization,
and Regulated Expression of Ig Genes. Mage 20-21
00227-01 Biochemistry of Lymphocyte Activation.
00228-01 Membrane Associated Glycoconjugates and Recep-
tors as Differentiation Markers and Mediators
of Cell-Cell Recognition and Communication.
00229-01 Studies on Lymphocyte Differentiation. Davis 20-24
00259-01 la Molecules and Immune Response Genes. Paul 20-25
Office of the Chief
Laboratory of Immunology
October 1, 1981 through September 30, 1982
The Laboratory of Immunology is concerned with the elucidation of the
fundamental mechanisms underlying immunologic responses. It has made Voltaren Emulgel Price rapid
progress through the use of three new technologies which are creating a revolu-
tion in immunologic sciences. These are the use of monoclonal antibodies, the
adaptation of techniques of molecular genetics to immunologic problems, and
the use of long-term lines of cloned normal and transformed lymphocytes. The
continued use and improvement of these approaches should allow solution of
many of the major problems which have concerned immunologists and should pro-
vide important approaches to the more precise regulation of the normal and the
disordered immune response.
Identification of Genes Uniquely Expressed in Lymphocytes of Specific Differen-
The phenotype of any individual cell depends upon those genes which Voltaren Emulgel Usa it
has expressed. To a large extent, the differences in function and structure
of two closely related cells are determined by the genes expressed in the mRNA
of one cell but not another. Laboratory Voltaren Emulgel 20g of Immunology scientists have examined
the expressed genetic differences in lymphoid cells of distinct differentiated
states by preparing cDNA probes which hybridize specifically with mRNA of one
but not another member of a pair of cells. For example, cDNA prepared from the
mRNA of a B cell tumor was extensively hybridized with mRNA from a T cell
tumor. The single stranded material represents a "B cell specific" probe
(B -T); similarly, T^-B Voltaren Emulgel Canada and T,^-T probes have been prepared. Based on the
hybridization kinetics of these probes, it has been estimated that B-T and T-B
differences are '^-1.7-2.4% of the total mRNA and correspond to about 200 dis-
tict genes expressed in one cell type but not the other. An analysis of the
difference in expressed genes between a helper T cell hybridoma line and a sup-
pressor T cell hybridoma line indicates that these differ from one another in
about 0.3% of their expressed genes, corresponding to approximately 40 differ-
ent genes. The number of these genes is sufficiently small that their isola-
tion and characterization should be feasible. Selected cDNA's (B -T and T -B)
have been cloned to prepare cell type-specific cDNA libraries. The availabil-
ity of such libraries, coupled with specific probes, should greatly simplify
the task of isolating each gene. Among the things such libraries have been
used for thus far is to identify X chromosomal genes of the B -T or T -B type.
This appeared particularly interesting because of the existence of multiple
distinct human and mouse immunodeficiencies controlled by X chromosomal genes.
One X chromosomal gene, Voltaren Emulgel 50g pTX-1 , identified in a T -B cDNA library, exists as a
gene family. This cDNA hybridizes with 10 or 12 restriction fragments from
the mouse X chromosome; pTX-1 appears to be expressed in T helper cells and
not T suppressor cells. Due to the importance of gene families among other
genetic systems involved in immune responses (i.e., immunoglobulins, Voltaren Emulgel 1.16 Class I MHC
molecules, y-interferons) , this X chromosomal set of genes merits considerable
attention; it may exert a critical role in lymphocyte differentiation (Davis,
Cohen, Hedrick, Heber-Katz, Schwartz and Paul, LI/NIAID; Kim and Asofsky, LMI/
NIAID; Hansburg, LCB/NCI ; D'Eustachio, Yale University).
Cloning and Characterization of cDNA containing DNA Sequences for Rabbit k Light
Chains and p Heavy Chains
The understanding of the organization and regulation of expression of rab-
bit immunoglobulin (Ig) genes may lead to important general Voltaren Emulgel 1 insights because
of several very interesting features of rabbit Igs, including the expression of
a^ locus allotypes Voltaren Emulgel Diclofenac Topical Gel on Igh-V regions, the phenomenon of hidden or latent allotypes
and the capacity to Voltaren Emulgel 120g induce long-term allotype suppression. In order to realize
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